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  <!-- Inheritance for Line Items:  us-gaap:StatementLineItems-->
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  <!-- Dimension Defaults -->
  <link:definitionLink xlink:type="extended" xlink:role="http://healthstream.com/role/CollaborativeArrangementDetails" xlink:title="0608 - Disclosure - Collaborative Arrangement (Details)">
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    <link:loc xlink:type="locator" xlink:href="hstm-20120630.xsd#hstm_TypeOfArrangementTransactionDomain" xlink:label="hstm_TypeOfArrangementTransactionDomain" />
    <link:definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TypeOfArrangementAxis" xlink:to="hstm_TypeOfArrangementTransactionDomain" order="1" />
  </link:definitionLink>
  <!-- Dimension Defaults -->
  <link:definitionLink xlink:type="extended" xlink:role="http://healthstream.com/role/CollaborativeArrangementDetails" xlink:title="0608 - Disclosure - Collaborative Arrangement (Details)">
    <link:loc xlink:type="locator" xlink:href="http://xbrl.sec.gov/dei/2012/dei-2012-01-31.xsd#dei_LegalEntityAxis" xlink:label="dei_LegalEntityAxis" />
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</link:linkbase>
</XBRL>
